Widefield imaging of rapid pan-cortical voltage dynamics with an indicator evolved for one-photon microscopy
Lu, X., Wang, Y., Liu, Z., Gou, Y., Jaeger, D., & St-Pierre, F. (2023). Widefield imaging of rapid pan-cortical voltage dynamics with an indicator evolved for one-photon microscopy. Nature Communications, 14(1), 6423.
Oct. 12, 2023
A genetically encoded voltage indicator, JEDI-1P enable wide brain imaging of fast neural activity.
Automating the high-throughput screening of protein-based optical indicators and actuators
Lee, J., Campillo, B., Hamidian, S., Liu, Z., Shorey, M., & St-Pierre, F. (2022). Automating the High-Throughput Screening of Protein-Based Optical Indicators and Actuators. Biochemistry, 62(2), 169-177.
Oct. 31, 2022
A review of protein engineering approaches to screen and optimize optical sensors and indicators for biological systems.
Sustained deep-tissue voltage recording using a fast indicator optimized for two-photon microscopy
Liu, Z., Lu, X., Villette, V., Gou, Y., Colbert, K. L., Lai, S., ... & St-Pierre, F. (2022). Sustained deep-tissue voltage recording using a fast indicator evolved for two-photon microscopy. Cell, 185(18), 3408-3425.
These authors contributed equally: Liu, Z., Lu, X., Villette, V., Gou, Y.
Aug. 18, 2022
Engineer a fast, sensitive, bright and photostable genetically encoded voltage indicator called JEDI-2P that is compatible with two-photon microscopy. JEDI-2P enables deep-tissue optical recording of rapid voltage dynamics over tens of minutes.
Lee, J., Liu, Z., Suzuki, P. H., Ahrens, J. F., Lai, S., Lu, X., ... & St-Pierre, F. (2020). Versatile phenotype-activated cell sorting. Science advances, 6(43), eabb7438.
Oct. 23, 2020
A versatile high-throughput technique called SPOTlight to isolate individual yeast or human cells with unique spatiotemporal profiles from heterogeneous populations. This technique allows engineer of a bright and new yellow fluorescent protein mGold.